Mast cell-specific Cre/loxP-mediated recombination in vivo

Scholten, Julia and Hartmann, Karin and Gerbaulet, Alexander and Krieg, Thomas and Müller, Werner and Testa, Giuseppe and Roers, Axel. (2008) Mast cell-specific Cre/loxP-mediated recombination in vivo. Transgenic Research, 17 (2). pp. 307-315.

Full text not available from this repository.

Official URL: https://edoc.unibas.ch/70832/

Downloads: Statistics Overview


Mast cells are important effectors of type I allergy but also essential regulators of innate and adaptive immune responses. The aim of this study was to develop a Cre recombinase-expressing mouse line that allows mast cell-specific inactivation of genes in vivo. Following a BAC transgenic approach, Cre was expressed under the control of the mast cell protease (Mcpt) 5 promoter. Mcpt5-Cre transgenic mice were crossed to the ROSA26-EYFP Cre excision reporter strain. Efficient Cre-mediated recombination was observed in mast cells from the peritoneal cavity and the skin while only minimal reporter gene expression was detected outside the mast cell compartment. Our results show that the Mcpt5 promoter can drive Cre expression in a mast cell-specific fashion. We expect that our Mcpt5-Cre mice will be a useful tool for the investigation of mast cell biology.
Faculties and Departments:03 Faculty of Medicine > Departement Biomedizin > Department of Biomedicine, University Hospital Basel > Allergy and Immunity (Hartmann)
UniBasel Contributors:Hartmann, Karin
Item Type:Article, refereed
Article Subtype:Research Article
Note:Publication type according to Uni Basel Research Database: Journal article
Related URLs:
Identification Number:
Last Modified:10 Nov 2020 14:44
Deposited On:10 Nov 2020 14:44

Repository Staff Only: item control page