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On-cell catalysis by surface engineering of live cells with an artificial metalloenzyme

Szponarski, Mathieu and Schwizer, Fabian and Ward, Thomas R. and Gademann, Karl. (2018) On-cell catalysis by surface engineering of live cells with an artificial metalloenzyme. Communications Chemistry, 1 (84). pp. 1-10.

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Official URL: https://edoc.unibas.ch/70532/

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Abstract

Metal-catalyzed chemical transformations performed at the cellular level bear great potential for the manipulation of biological processes. The complexity of the cell renders the use of transition metal chemistry difficult in cellular systems. The delivery of the reactive catalyst and the control of its spatial localization remain challenging. Here we report the surface functionalization of the unicellular eukaryote Chlamydomonas reinhardtii with a tailor-made artificial metalloenzyme for on-cell catalysis. The functionalized cells remain viable and are able to uncage a fluorogenic substrate on their surface. This work leverages cell surface engineering to provide live cells with new-to-nature reactivity. In addition, this operationally simple approach is not genetically encoded and thereby transient, which offers advantages with regard to temporal control, cell viability, and safety. Therefore, and as a feature, the movement of the functionalized cells can be directed by light (via phototaxis), allowing for the three-dimensional localization of catalysts by outside stimuli.
Faculties and Departments:05 Faculty of Science > Departement Chemie > Chemie > Bioanorganische Chemie (Ward)
UniBasel Contributors:Ward, Thomas R.R. and Schwizer, Fabian and Gademann, Karl
Item Type:Article, refereed
Article Subtype:Research Article
Publisher:Nature Research
e-ISSN:2399-3669
Note:Publication type according to Uni Basel Research Database: Journal article
Language:English
Identification Number:
Last Modified:22 May 2019 08:03
Deposited On:22 May 2019 07:54

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