Pushing the mass limit for intact launch and photoionization of large neutral biopolymers

Schätti, Jonas and Rieser, Philipp Rieser and Sezer, Ugur and Richter, Georg and Geyer, Philipp and Rondina, Gustavo G. and Häussinger, Daniel and Mayor, Marcel and Shayeghi, Armin and Köhler, Valentin and Arndt, Markus. (2018) Pushing the mass limit for intact launch and photoionization of large neutral biopolymers. Communications Chemistry, 1. p. 93.

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Since their first discovery by Louis Dunoyer and Otto Stern, molecular beams have conquered research and technology. However, it has remained an outstanding challenge to isolate and photoionize beams of massive neutral polypeptides. Here we show that femtosecond desorption from a matrix-free sample in high vacuum can produce biomolecular beams at least 25 times more efficiently than nanosecond techniques. While it has also been difficult to photoionize large biomolecules, we find that tailored structures with an abundant exposure of tryptophan residues at their surface can be ionized by vacuum ultraviolet light. The combination of these desorption and ionization techniques allows us to observe molecular beams of neutral polypeptides with a mass exceeding 20,000 amu. They are composed of 50 amino acids – 25 tryptophan and 25 lysine residues – and 26 fluorinated alkyl chains. The tools presented here offer a basis for the preparation, control and detection of polypeptide beams.
Faculties and Departments:05 Faculty of Science > Departement Chemie
05 Faculty of Science > Departement Chemie > Chemie > Nuclear Magnetic Resonance (Häussinger)
05 Faculty of Science > Departement Chemie > Chemie > Bioanorganische Chemie (Ward)
UniBasel Contributors:Mayor, Marcel and Schätti, Jonas and Köhler, Valentin and Häussinger, Daniel
Item Type:Article, refereed
Article Subtype:Research Article
Publisher:Nature Publishing Group
Note:Publication type according to Uni Basel Research Database: Journal article
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Last Modified:03 Apr 2023 09:45
Deposited On:03 Jul 2019 15:03

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