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Use of multicellular tumor spheroids to dissect endothelial cell-tumor cell interactions: A role for T-cadherin in tumor angiogenesis

Ghosh, Sourabh and Josh, Manjunath B. and Ivanov, Danila and Feder-Mengus, Chantal and Spagnoli, Giulio C. and Martin, Ivan and Erne, Paul and Resink, Therese J.. (2007) Use of multicellular tumor spheroids to dissect endothelial cell-tumor cell interactions: A role for T-cadherin in tumor angiogenesis. FEBS letters, Vol. 581, H. 23. pp. 4523-4528.

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Official URL: http://edoc.unibas.ch/dok/A5248963

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Abstract

This study addresses establishment of an ``in vitro`` melanoma angiogenesis model using multicellular tumor spheroids (MCTS) of differentiated (HBL) or undifferentiated (NA8) melanoma cell lines. DNA microarray assay and qRTPCR indicated upregulation of pro-angiogenic factors IL-8, VEGF, Ephrin A] and ANGPTL4 in NA8-MCTSs (vs. monolayers) whereas these were absent in MCTS and monolayer cultures of HBL. Upon co-culture with endothelial cell line HMEC-1 NA8-MCTS attract, whereas HBL-MCTS repulse, HMEC-1. Overexpression of T-cadherin in HMEC-1 leads to their increased invasion and network formation within NA8-MCTS. Given an appropriate angiogenic tumor micro-environment, T-cadherin upregulation on endothelial cells may potentiate intratumoral angiogenesis. (C) 2007 Published by Elsevier B.V. on behalf of the Federation of European Biochemical Societies.
Faculties and Departments:03 Faculty of Medicine > Departement Biomedizin > Department of Biomedicine, University Hospital Basel > Tissue Engineering (Martin)
UniBasel Contributors:Martin, Ivan
Item Type:Article, refereed
Article Subtype:Research Article
Bibsysno:Link to catalogue
Publisher:Elsevier Science
ISSN:0014-5793
Note:Publication type according to Uni Basel Research Database: Journal article
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Last Modified:22 Mar 2012 14:23
Deposited On:22 Mar 2012 13:35

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