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Enzyme-Mimetic Antioxidant Luminescent Nanoparticles for Highly Sensitive Hydrogen Peroxide Biosensing

Pratsinis, Anna and Kelesidis, Georgios A. and Zuercher, Stefanie and Krumeich, Frank and Bolisetty, Sreenath and Mezzenga, Raffaele and Leroux, Jean-Christophe and Sotiriou, Georgios A.. (2017) Enzyme-Mimetic Antioxidant Luminescent Nanoparticles for Highly Sensitive Hydrogen Peroxide Biosensing. ACS nano, 11 (12). pp. 12210-12218.

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Official URL: https://edoc.unibas.ch/64196/

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Abstract

Hydrogen peroxide (H2O2) is an abundant molecule associated with biological functions and reacts with natural enzymes, such as catalase. Even though direct H2O2 measurement can be used to diagnose pathological conditions, such as infection and inflammation, H2O2 quantification further enables the detection of disease biomarkers in enzyme-linked assays (e.g. ELISA) in which enzymatic reactions may generate or consume H2O2. Such a quantification is often measured optically with organic dyes in biological media that suffer, however, from poor stability. Currently, the optical H2O2 biosensing without organic-dyes in biological media and at low, submicromolar, concentrations has yet to be achieved. Herein, we rationally design biomimetic artificial enzymes based on antioxidant CeO2 nanoparticles that become luminescent upon their Eu3+ doping. We vary systematically their diameter from 4 to 16 nm and study their catalase-mimetic antioxidant activity, manifested as catalytic H2O2 decomposition in aqueous solutions, revealing a strong nanoparticle surface area dependency. The interaction with H2O2 influences distinctly the particle luminescence rendering them highly sensitive H2O2 biosensors down to 0.15 μM (5.2 ppb) in solutions for biological assays. Our results link two, so far, unrelated research domains, the CeO2 nanoparticle antioxidant activity and luminescence by rare-earth doping. When these enzyme-mimetic nanoparticles are coupled with alcohol oxidase, biosensing can be extended to ethanol exemplifying how their detection potential can be broadened to additional biologically relevant metabolites. The enzyme-mimetic nanomaterial developed here could serve as a starting point of sophisticated in vitro assays toward the highly sensitive detection of disease biomarkers.
Faculties and Departments:05 Faculty of Science > Departement Pharmazeutische Wissenschaften > Pharmazie > Pharmazeutische Technologie (Huwyler)
UniBasel Contributors:Pratsinis, Anna
Item Type:Article, refereed
Article Subtype:Research Article
ISSN:1936-086X
Note:Publication type according to Uni Basel Research Database: Journal article
Identification Number:
Last Modified:23 Feb 2019 13:10
Deposited On:23 Feb 2019 13:10

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