Efficient transgenesis and annotated genome sequence of the regenerative flatworm model; Macrostomum lignano

Wudarski, Jakub and Simanov, Daniil and Ustyantsev, Kirill and De Mulder, Katrien and Grelling, Margriet and Grudniewska, Magda and Beltman, Frank and Glazenburg, Lisa and Demircan, Turan and Wunderer, Julia and Qi, Weihong and Vizoso , Dita B. and Weissert, Philipp M. and Olivieri, Mario and Mouton, Stijn and Guryev, Victor and Aboobaker, Victor and Schärer, Lukas and Ladurner, Peter and Berezikov, Eugene. (2017) Efficient transgenesis and annotated genome sequence of the regenerative flatworm model; Macrostomum lignano. Nature Communications, 8. p. 2120.

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Regeneration-capable flatworms are informative research models to study the mechanisms of stem cell regulation, regeneration, and tissue patterning. However, the lack of transgenesis methods considerably hampers their wider use. Here we report development of a transgenesis method for Macrostomum lignano, a basal flatworm with excellent regeneration capacity. We demonstrate that microinjection of DNA constructs into fertilized one-cell stage eggs, followed by a low dose of irradiation, frequently results in random integration of the transgene in the genome and its stable transmission through the germline. To facilitate selection of promoter regions for transgenic reporters, we assembled and annotated the M. lignano genome, including genome-wide mapping of transcription start regions, and show its utility by generating multiple stable transgenic lines expressing fluorescent proteins under several tissue-specific promoters. The reported transgenesis method and annotated genome sequence will permit sophisticated genetic studies on stem cells and regeneration using M. lignano as a model organism.
Faculties and Departments:05 Faculty of Science > Departement Umweltwissenschaften > Integrative Biologie > Evolutionary Biology (Schärer)
UniBasel Contributors:Schärer, Lukas
Item Type:Article, refereed
Article Subtype:Research Article
Publisher:Nature Research
Note:Publication type according to Uni Basel Research Database: Journal article
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Last Modified:12 Apr 2022 08:56
Deposited On:07 Sep 2020 08:32

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