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Monitoring single-cell gene regulation under dynamically controllable conditions with integrated microfluidics and software.

Kaiser, Matthias and Jug, Florian and Julou, Thomas and Deshpande, Siddharth and Pfohl, Thomas and Silander, Olin K. and Myers, Gene and van Nimwegen, Erik. (2018) Monitoring single-cell gene regulation under dynamically controllable conditions with integrated microfluidics and software. Nature Communications (9). p. 212.

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Abstract

Much is still not understood about how gene regulatory interactions control cell fate decisions in single cells, in part due to the difficulty of directly observing gene regulatory processes in vivo. We introduce here a novel integrated setup consisting of a microfluidic chip and accompanying analysis software that enable long-term quantitative tracking of growth and gene expression in single cells. The dual-input Mother Machine (DIMM) chip enables controlled and continuous variation of external conditions, allowing direct observation of gene regulatory responses to changing conditions in single cells. The Mother Machine Analyzer (MoMA) software achieves unprecedented accuracy in segmenting and tracking cells, and streamlines high-throughput curation with a novel leveraged editing procedure. We demonstrate the power of the method by uncovering several novel features of an iconic gene regulatory program: the induction of Escherichia coli's lac operon in response to a switch from glucose to lactose.
Faculties and Departments:05 Faculty of Science > Departement Biozentrum > Computational & Systems Biology > Bioinformatics (van Nimwegen)
UniBasel Contributors:van Nimwegen, Erik and Kaiser, Matthias and Julou, Thomas and Silander, Olin
Item Type:Article
Article Subtype:Research Article
Publisher:Nature Publishing Group
e-ISSN:2041-1723
Language:English
Identification Number:
Last Modified:19 Oct 2018 14:07
Deposited On:19 Oct 2018 14:07

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