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Second messenger-mediated tactile response by a bacterial rotary motor

Hug, Isabelle and Deshpande, Siddharth and Sprecher, Kathrin S. and Pfohl, Thomas and Jenal, Urs. (2017) Second messenger-mediated tactile response by a bacterial rotary motor. Science, 358 (6362). pp. 531-534.

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Official URL: https://edoc.unibas.ch/59162/

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Abstract

When bacteria encounter surfaces, they respond with surface colonization and virulence induction. The mechanisms of bacterial mechanosensation and downstream signaling remain poorly understood. Here, we describe a tactile sensing cascade in Caulobacter crescentus in which the flagellar motor acts as sensor. Surface-induced motor interference stimulated the production of the second messenger cyclic diguanylate by the motor-associated diguanylate cyclase DgcB. This led to the allosteric activation of the glycosyltransferase HfsJ to promote rapid synthesis of a polysaccharide adhesin and surface anchoring. Although the membrane-embedded motor unit was essential for surface sensing, mutants that lack external flagellar structures were hypersensitive to mechanical stimuli. Thus, the bacterial flagellar motor acts as a tetherless sensor reminiscent of mechanosensitive channels.
Faculties and Departments:05 Faculty of Science > Departement Biozentrum > Infection Biology > Molecular Microbiology (Jenal)
05 Faculty of Science > Departement Biozentrum > Growth & Development > Molecular Microbiology (Jenal)
05 Faculty of Science > Departement Biozentrum > Infection Biology
UniBasel Contributors:Jenal, Urs and Hug, Isabelle and Sprecher, Kathrin
Item Type:Article, refereed
Article Subtype:Research Article
Publisher:American Association for the Advancement of Science
ISSN:0036-8075
e-ISSN:1095-9203
Note:Publication type according to Uni Basel Research Database: Journal article -- The final publication is available at American Association for the Advancement of Science see DOI link
Language:English
Identification Number:
Last Modified:26 Jan 2018 15:50
Deposited On:26 Jan 2018 15:50

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