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Immobilization of Enzymes on PLGA Sub-Micrometer Particles by Crosslinked Layer-by-Layer Deposition

Sieber, Sandro and Siegrist, Stefan and Schwarz, Stéphanie and Porta, Fabiola and Schenk, Susanne H. and Huwyler, Jörg. (2017) Immobilization of Enzymes on PLGA Sub-Micrometer Particles by Crosslinked Layer-by-Layer Deposition. Macromolecular Bioscience, 17 (8). p. 1700015.

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Official URL: http://edoc.unibas.ch/54926/

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Abstract

Enzyme immobilization is of high interest for industrial applications. However, immobilization may compromise enzyme activity or stability due to the harsh conditions which have to be applied. The authors therefore present a new and improved crosslinked layer-by-layer (cLbL) approach. Two different model enzymes (acid phosphatase and beta-galactosidase) are immobilized under mild conditions on biocompatible, monodisperse, sub-micrometer poly(lactide-coglycolide) (PLGA) particles. The resulting PLGA enzyme systems are characterized regarding their size, surface charge, enzyme activity, storage stability, reusability, and stability under various conditions such as changing pH and temperature. The developed and characterized cLbL protocol can be easily adapted to different enzymes. Potential future uses of the technology for biomedical applications are discussed. PLGA-enzyme particles are therefore injected into the blood circulation of zebrafish embryos in order to demonstrate the in vivo stability and activity of the designed system.
Faculties and Departments:05 Faculty of Science > Departement Pharmazeutische Wissenschaften > Pharmazie > Pharmazeutische Technologie (Huwyler)
UniBasel Contributors:Huwyler, Jörg and Sieber, Sandro and Siegrist, Stefan and Porta, Fabiola and Schenk, Susanne
Item Type:Article, refereed
Article Subtype:Research Article
Publisher:Wiley
ISSN:1616-5187
e-ISSN:1616-5195
Note:Publication type according to Uni Basel Research Database: Journal article
Identification Number:
Last Modified:24 Oct 2017 07:22
Deposited On:10 Oct 2017 08:40

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