Kornilova, S. V. and Iasem, P. and Grigor'ev, D. N. and Kapinos, L. E. and Totova, Ia and Blagoĭ, Iu P.. (1997) Kalorimetricheskoe issledovanie vliianiia ionov Ca2+ i Mn2+ na perekhodspiral'-klubok v DNK. Biofizika, 42 (3). pp. 599-606.
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Official URL: http://edoc.unibas.ch/48622/
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Abstract
Using the method of differential scanning calorimetry, the DNA helix-coil transition studied in solutions containing divalent metal ions (Mn2+ and Ca2+) at relative metal ion concentrations (Me/PDNA) ranging from 0.2 to 20. Dependences of the melting temperature and enthalpy on the ion relative concentration were measured. The fine structure of melting curves in DNA+Me complexes was observed. The resolution degree of this structure was shown to increase in the range of moderate ion concentrations. The data obtained were compared with values determined by UV-spectroscopy. Association constants were defined for Mn2+ and Ca2+ binding to DNA by the ligand theory.
| Faculties and Departments: | 05 Faculty of Science > Departement Biozentrum > Structural Biology & Biophysics > Nanobiology Argovia (Lim) |
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| UniBasel Contributors: | Kapinos Schneider, Larisa E. E |
| Item Type: | Article, refereed |
| Article Subtype: | Research Article |
| Publisher: | Izdatel'stvo Nauka |
| ISSN: | 0006-3029 |
| Note: | Publication type according to Uni Basel Research Database: Journal article |
| Identification Number: |
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| Last Modified: | 10 Nov 2017 10:27 |
| Deposited On: | 10 Nov 2017 10:27 |
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