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Kalorimetricheskoe issledovanie vliianiia ionov Ca2+ i Mn2+ na perekhodspiral'-klubok v DNK

Kornilova, S. V. and Iasem, P. and Grigor'ev, D. N. and Kapinos, L. E. and Totova, Ia and Blagoĭ, Iu P.. (1997) Kalorimetricheskoe issledovanie vliianiia ionov Ca2+ i Mn2+ na perekhodspiral'-klubok v DNK. Biofizika, 42 (3). pp. 599-606.

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Official URL: http://edoc.unibas.ch/48622/

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Abstract

Using the method of differential scanning calorimetry, the DNA helix-coil transition studied in solutions containing divalent metal ions (Mn2+ and Ca2+) at relative metal ion concentrations (Me/PDNA) ranging from 0.2 to 20. Dependences of the melting temperature and enthalpy on the ion relative concentration were measured. The fine structure of melting curves in DNA+Me complexes was observed. The resolution degree of this structure was shown to increase in the range of moderate ion concentrations. The data obtained were compared with values determined by UV-spectroscopy. Association constants were defined for Mn2+ and Ca2+ binding to DNA by the ligand theory.
Faculties and Departments:05 Faculty of Science > Departement Biozentrum > Structural Biology & Biophysics > Nanobiology Argovia (Lim)
UniBasel Contributors:Kapinos Schneider, Larisa E. E
Item Type:Article, refereed
Article Subtype:Research Article
Publisher:Izdatel'stvo Nauka
ISSN:0006-3029
Note:Publication type according to Uni Basel Research Database: Journal article
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Last Modified:10 Nov 2017 10:27
Deposited On:10 Nov 2017 10:27

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