edoc

Simple biophysics underpins collective conformations of the intrinsically disordered proteins of the Nuclear Pore Complex

Vovk, Andrei and Gu, Chad and Opferman, Michael G. and Kapinos, Larisa E. and Lim, Roderick Y. H. and Coalson, Rob D. and Jasnow, David and Zilman, Anton. (2016) Simple biophysics underpins collective conformations of the intrinsically disordered proteins of the Nuclear Pore Complex. eLife, 5. e10785.

[img] PDF - Published Version
Available under License CC BY (Attribution).

3765Kb

Official URL: http://edoc.unibas.ch/43200/

Downloads: Statistics Overview

Abstract

Nuclear Pore Complexes (NPCs) are key cellular transporter that control nucleocytoplasmic transport in eukaryotic cells, but its transport mechanism is still not understood. The centerpiece of NPC transport is the assembly of intrinsically disordered polypeptides, known as FG nucleoporins, lining its passageway. Their conformations and collective dynamics during transport are difficult to assess in vivo. In vitro investigations provide partially conflicting results, lending support to different models of transport, which invoke various conformational transitions of the FG nucleoporins induced by the cargo-carrying transport proteins. We show that the spatial organization of FG nucleoporin assemblies with the transport proteins can be understood within a first principles biophysical model with a minimal number of key physical variables, such as the average protein interaction strengths and spatial densities. These results address some of the outstanding controversies and suggest how molecularly divergent NPCs in different species can perform essentially the same function.
Faculties and Departments:05 Faculty of Science > Departement Biozentrum > Structural Biology & Biophysics > Nanobiology Argovia (Lim)
UniBasel Contributors:Lim, Roderick Y.H. and Kapinos Schneider, Larisa E. E
Item Type:Article, refereed
Article Subtype:Research Article
Publisher:eLife Sciences Publications
ISSN:2050-084X
Note:Publication type according to Uni Basel Research Database: Journal article
Language:English
Identification Number:
Last Modified:29 Sep 2016 09:33
Deposited On:29 Sep 2016 09:33

Repository Staff Only: item control page