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Biological, serological, and molecular characterization of a hghly divergent strain of grapevine leafroll-associated virus 4 causing grapevine leafroll disease

Reynard, Jean-Sébastien and Schneeberger, Pierre H. H. and Frey, Jürg Ernst and Schaerer, Santiago. (2015) Biological, serological, and molecular characterization of a hghly divergent strain of grapevine leafroll-associated virus 4 causing grapevine leafroll disease. Phytopathology, 105 (9). pp. 1262-1269.

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Official URL: http://edoc.unibas.ch/41203/

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Abstract

The complete genome sequence of a highly divergent strain of Grapevine leafroll-associated virus 4 (GLRaV-4) was determined using 454 pyrosequencing technology. This virus, designated GLRaV-4 Ob, was detected in Vitis vinifera 'Otcha bala' from our grapevine virus collection at Agroscope. The GLRaV-4 Ob genome length and organization share similarities with members of subgroup II in the genus Ampelovirus (family Closteroviridae). Otcha bala was graft-inoculated onto indicator plants of cultivar Gamay to evaluate the biological properties of this new strain, and typical leafroll symptoms were induced. A monoclonal antibody for the rapid detection of GLRaV-4 Ob by enzyme-linked immunosorbent assay is available, thus facilitating large-scale diagnostics of this virus. Based on the relatively small size of the coat protein, the reduced amino acid identity and the distinct serological properties, our study clearly shows that GLRaV-4 Ob is a divergent strain of GLRaV-4. Furthermore, molecular and serological data revealed that the AA42 accession from which GLRaV-7 was originally reported is in fact co-infected with GLRaV-4 Ob and GLRaV-7. This finding challenges the idea that GLRaV-7 is a leafroll-causing agent.
Faculties and Departments:09 Associated Institutions > Swiss Tropical and Public Health Institute (Swiss TPH)
09 Associated Institutions > Swiss Tropical and Public Health Institute (Swiss TPH) > Department of Epidemiology and Public Health (EPH) > Eco System Health Sciences > Health Impact Assessment (Utzinger)
UniBasel Contributors:Schneeberger, Pierre
Item Type:Article, refereed
Article Subtype:Research Article
Publisher:American Phytopathological Society
ISSN:0031-949X
Note:Publication type according to Uni Basel Research Database: Journal article
Identification Number:
Last Modified:30 Jun 2016 11:02
Deposited On:11 Apr 2016 12:37

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