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Optimization of GFP levels for analyzing Salmonella gene expression during an infection

Wendland, M. and Bumann, D.. (2002) Optimization of GFP levels for analyzing Salmonella gene expression during an infection. FEBS Letters, 521 (1-3). pp. 105-108.

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Official URL: http://edoc.unibas.ch/dok/A5259825

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Abstract

Green fluorescent protein (GFP) is an attractive reporter for Salmonella gene expression analysis but might interfere with virulence when expressed at high levels. To identify suitable GFP levels, we constructed a series of Salmonella strains expressing different amounts of GFP and measured their fluorescence and colonization levels in infected mice. The results show that GFP concentrations in the range of 7000-200,000 molecules per Salmonella cell are detectable in ex vivo samples using flow cytometry, and cause no major Salmonella virulence defect. Appropriate GFP levels can be obtained with weak promoters and stable GFP, or strong promoters and destabilized GFP.
Faculties and Departments:05 Faculty of Science > Departement Biozentrum > Infection Biology > Molecular Microbiology (Bumann)
UniBasel Contributors:Bumann, Dirk
Item Type:Article, refereed
Article Subtype:Research Article
Bibsysno:Link to catalogue
Publisher:Elsevier
ISSN:0014-5793
e-ISSN:1873-3468
Note:Publication type according to Uni Basel Research Database: Journal article
Identification Number:
Last Modified:27 Nov 2017 07:27
Deposited On:22 Mar 2012 13:23

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