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Rapidly maturing red fluorescent protein variants with strongly enhanced brightness in bacteria

Sorensen, M. and Lippuner, C. and Kaiser, T. and Misslitz, A. and Aebischer, T. and Bumann, D.. (2003) Rapidly maturing red fluorescent protein variants with strongly enhanced brightness in bacteria. FEBS Letters, 552 (2-3). pp. 110-114.

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Official URL: http://edoc.unibas.ch/dok/A5259818

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Abstract

A rapidly maturing variant of the red fluorescent protein DsRed was optimized for bacterial expression by random mutagenesis. The brightest variant contains six mutations, two of which (S4T and a silent mutation in codon 2) explain most of the fluorescence enhancement. The novel variants are expressed at 9-60-fold higher levels in Escherichia coli compared to DsRed.T3, but are not superior fluorophores on a per molecule basis. In contrast to previously available DsRed variants, DsRed.T3_S4T is sufficiently bright to monitor Salmonella gene expression in infected animals using flow cytometry. However, no fluorescence enhancement was observed in Leishmania or HeLa cells, indicating that these novel variants are specifically useful for bacteria.
Faculties and Departments:05 Faculty of Science > Departement Biozentrum > Infection Biology > Molecular Microbiology (Bumann)
UniBasel Contributors:Bumann, Dirk
Item Type:Article, refereed
Article Subtype:Research Article
Bibsysno:Link to catalogue
Publisher:Elsevier
ISSN:0014-5793
e-ISSN:1873-3468
Note:Publication type according to Uni Basel Research Database: Journal article
Identification Number:
Last Modified:27 Nov 2017 07:26
Deposited On:22 Mar 2012 13:23

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