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The role of 3D structure and protein conformation on the innate and adaptive immune responses to silk-based biomaterials

Bhattacharjee, M. and Schultz-Thater, E. and Trella, E. and Miot, S. and Das, S. and Loparic, M. and Ray, A. R. and Martin, I. and Spagnoli, G. C. and Ghosh, S.. (2013) The role of 3D structure and protein conformation on the innate and adaptive immune responses to silk-based biomaterials. Biomaterials, Vol. 34, no. 33. pp. 8161-8171.

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Official URL: http://edoc.unibas.ch/dok/A6338097

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Abstract

We have investigated monocyte and T cell responsiveness to silk based biomaterials of different physico-chemical characteristics. Here we report that untransformed CD14+ human monocytes respond to overnight exposure to silk fibroin-based biomaterials in tridimensional form by IL-1beta and IL-6, but not IL-10 gene expression and protein production. In contrast, fibroin based materials in bidimensional form are unable to stimulate monocyte responsiveness. The elicitation of these effects critically requires contact between biomaterials and responding cells, is not sustained and becomes undetectable in longer term cultures. We also observed that NF-kappabeta and p38 MAP kinase play key roles in monocyte activation by silk-based biomaterials. On the other hand, fibroin based materials, irrespective of their physico-chemical characteristics appeared to be unable to induce the activation of peripheral blood T cells from healthy donors, as evaluated by the expression of activation markers and IFN-gamma gene.
Faculties and Departments:03 Faculty of Medicine > Departement Biomedizin > Department of Biomedicine, University Hospital Basel > Oncology Surgery (Spagnoli)
03 Faculty of Medicine > Departement Biomedizin > Department of Biomedicine, University Hospital Basel > Tissue Engineering (Martin)
UniBasel Contributors:Spagnoli, Giulio C. and Martin, Ivan
Item Type:Article, refereed
Article Subtype:Research Article
Bibsysno:Link to catalogue
Publisher:Elsevier
ISSN:0142-9612
Note:Publication type according to Uni Basel Research Database: Journal article
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Last Modified:06 Mar 2015 07:44
Deposited On:06 Mar 2015 07:44

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