edoc

Identification of tumor-specific Salmonella Typhimurium promoters and their regulatory logic

Leschner, Sara and Deyneko, Igor V. and Lienenklaus, Stefan and Wolf, Kathrin and Bloecker, Helmut and Bumann, Dirk and Loessner, Holger and Weiss, Siegfried. (2012) Identification of tumor-specific Salmonella Typhimurium promoters and their regulatory logic. Nucleic Acids Research, 40 (7). pp. 2984-2994.

[img]
Preview
PDF - Published Version
Available under License CC BY-NC (Attribution-NonCommercial).

5Mb

Official URL: http://edoc.unibas.ch/dok/A5848687

Downloads: Statistics Overview

Abstract

Conventional cancer therapies are often limited in effectiveness and exhibit strong side effects. Therefore, alternative therapeutic strategies are demanded. The employment of tumor-colonizing bacteria that exert anticancer effects is such a novel approach that attracts increasing attention. For instance, Salmonella enterica serovar Typhimurium has been used in many animal tumor models as well as in first clinical studies. These bacteria exhibit inherent tumoricidal effects. In addition, they can be used to deliver therapeutic agents. However, bacterial expression has to be restricted to the tumor to prevent toxic substances from harming healthy tissue. Therefore, we screened an S. Typhimurium promoter-trap library to identify promoters that exclusively drive gene expression in the cancerous tissue. Twelve elements could be detected that show reporter gene expression in tumors but not in spleen and liver. In addition, a DNA motif was identified that appears to be necessary for tumor specificity. Now, such tumor-specific promoters can be used to safely express therapeutic proteins by tumor-colonizing S. Typhimurium directly in the neoplasia.
Faculties and Departments:05 Faculty of Science > Departement Biozentrum > Infection Biology > Molecular Microbiology (Bumann)
UniBasel Contributors:Bumann, Dirk
Item Type:Article, refereed
Article Subtype:Research Article
Bibsysno:Link to catalogue
Publisher:Oxford University Press
ISSN:0305-1048
e-ISSN:1362-4962
Note:Publication type according to Uni Basel Research Database: Journal article
Language:English
Related URLs:
Identification Number:
Last Modified:22 Nov 2017 09:15
Deposited On:14 Sep 2012 06:38

Repository Staff Only: item control page