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Nuclear pore complex structure and plasticity revealed by electron and atomic force microscopy

Maco, Bohumil and Fahrenkrog, Birthe and Huang, Ning-Ping and Aebi, Ueli. (2006) Nuclear pore complex structure and plasticity revealed by electron and atomic force microscopy. Methods in Molecular Biology, Vol. 322. pp. 273-288.

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Official URL: http://edoc.unibas.ch/dok/A5259602

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Abstract

To study the ultrastructure of nuclear pore complexes (NPCs), a wide spectrum of different electron microscopy (EM) or atomic force microscopy (AFM) techniques can be employed. The combination of these methods can reveal new insights into the structural and functional organization of this important supramolecular machine through which nucleocytoplasmic transport occurs. Negative staining, quick freezing/freeze-drying/rotary metal shadowing, embedding and thin sectioning, cryoelectron microscopy and tomography, scanning electron microscopy, or combination with immunolabeling techniques are tools for collecting data and information about the three-dimensional structure and architecture of the NPCs. AFM enables investigation of the functional dynamics of native NPCs under physiological conditions.
Faculties and Departments:05 Faculty of Science > Departement Biozentrum > Former Organization Units Biozentrum > Structural Biology (Aebi)
05 Faculty of Science > Departement Biozentrum > Former Organization Units Biozentrum > Structural Biology (Fahrenkrog)
UniBasel Contributors:Aebi, Ueli and Fahrenkrog, Birthe
Item Type:Article, refereed
Article Subtype:Research Article
Bibsysno:Link to catalogue
Publisher:Humana Press
ISSN:1064-3745
e-ISSN:1940-6029
Note:Publication type according to Uni Basel Research Database: Journal article
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Last Modified:12 Oct 2017 10:01
Deposited On:22 Mar 2012 13:59

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