Arheit, Marcel and Castaño-Diéz, Daniel and Thierry, Raphaël and Abeyrathne, Priyanka and Gipson, Bryant R. and Stahlberg, Henning. (2013) Merging of image data in electron crystallography. Methods in Molecular Biology, 955. pp. 195-209.
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Official URL: http://edoc.unibas.ch/dok/A6056065
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Abstract
Electron crystallography of membrane proteins uses cryo-transmission electron microscopy to record images and diffraction patterns of frozen-hydrated 2D crystals. Each two-dimensional (2D) crystal is only imaged once, at one specific tilt angle, and the recorded images can be automatically processed with the 2dx/MRC software package. Processed image data from non-tilted and tilted 2D crystals then need to be merged into a 3D reconstruction of the membrane protein structure. We here describe the process of the 3D merging, using the 2dx software system.
| Faculties and Departments: | 05 Faculty of Science > Departement Biozentrum > Former Organization Units Biozentrum > Structural Biology (Stahlberg) |
|---|---|
| UniBasel Contributors: | Stahlberg, Henning |
| Item Type: | Article, refereed |
| Article Subtype: | Research Article |
| Publisher: | Humana Press |
| ISSN: | 1064-3745 |
| e-ISSN: | 1940-6029 |
| Note: | Publication type according to Uni Basel Research Database: Journal article |
| Related URLs: | |
| Identification Number: |
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| Last Modified: | 12 Oct 2017 10:05 |
| Deposited On: | 04 Jan 2013 08:37 |
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