Philippsen, Ansgar and Schirmer, Tilman and Stein, Martin A. and Giffhorn, Friedrich and Stetefeld, Jörg. (2005) Structure of zinc-independent sorbitol dehydrogenase from Rhodobacter sphaeroides at 2.4 A resolution. Acta Crystallographica. Section D, Biological Crystallography, Vol. 61, Pt. 4. pp. 374-379.
Full text not available from this repository.
Official URL: http://edoc.unibas.ch/dok/A5258247
Downloads: Statistics Overview
Abstract
Recombinant sorbitol dehydrogenase (SDH) from Rhodobacter sphaeroides has been crystallized in the absence of the cofactor NAD(H) and its structure determined to 2.4 A resolution using molecular replacement (refined R and R free factors of 18.8 and 23.8%, respectively). As expected from the sequence and shown by the conserved fold, SDH can be assigned to the short-chain dehydrogenase/reductase protein family. The cofactor NAD and the substrate sorbitol have been modelled into the structure and the active-site architecture, which displays the highly conserved catalytic tetrad of Asn-Ser-Tyr-Lys residues, is discussed in relation to the enzyme mechanism. This is the first structure of a bacterial SDH belonging to the SDR family.
| Faculties and Departments: | 05 Faculty of Science > Departement Biozentrum > Former Organization Units Biozentrum > Structural Biology (Schirmer) |
|---|---|
| UniBasel Contributors: | Schirmer, Tilman |
| Item Type: | Article, refereed |
| Article Subtype: | Research Article |
| Publisher: | Munksgaard |
| ISSN: | 0907-4449 |
| Note: | Publication type according to Uni Basel Research Database: Journal article |
| Last Modified: | 07 Aug 2015 12:05 |
| Deposited On: | 22 Mar 2012 13:20 |
Repository Staff Only: item control page