Cottenye, Nicolas. Antimicrobial surfaces based on self-assembled nanoreactors : from block copolymer synthesis to bacterial adhesion study. 2010, PhD Thesis, University of Basel, Faculty of Science.
Official URL: http://edoc.unibas.ch/diss/DissB_9317
Using E.coli strains, we first observed an influence of the two density of oligonucleotides immobilized on the surface on the number of adherent bacteria. This influence may be due to an effect of surface charge density. We then confirmed the well-known role of curli in biofilm cohesion, and we showed gene over-expression associated with curli production on oligonucleotide-modified surfaces. We demonstrated that gene over-expression does not depend on the topographical features of the surface or on the composition of the nucleotide sequences used in this study. Finally, we demonstrated that the presence of the vesicular structure is able to produce strong anti-adhesive properties of the surface. We assume, from observations of bacterial response in dynamic conditions, that this effect is due to increased bacterial motility on the surface, leading to a high detachment rate. Which is further confirms by a comparable bacterial response observed on agar hydrogel of different hardnesses. This result provides a preliminary outcome, paving the way to new approaches to antimicrobial strategies.
|Advisors:||Meier, Wolfgang P.|
|Committee Members:||Textor, Marcus|
|Faculties and Departments:||05 Faculty of Science > Departement Chemie > Chemie > Makromolekulare Chemie (Meier)|
|Bibsysno:||Link to catalogue|
|Number of Pages:||173 S.|
|Last Modified:||30 Jun 2016 10:41|
|Deposited On:||26 Jan 2011 14:10|
Repository Staff Only: item control page