Items where Author is "Humbert, Nicolas"

Article

Rusbandi, Untung E. and Lo, Cheikh and Skander, Myriem and Ivanova, Anita and Creus, Marc and Humbert, Nicolas and Ward, Thomas R.. (2007) Second Generation Artificial Hydrogenases Based on the Biotin-Avidin Technology: Improving Activity, Stability and Selectivity by Introduction of Enantiopure Amino Acid Spacers. Advanced Synthesis and Catalysis, 349 (11/12). pp. 1923-1930.

Letondor, Christophe and Pordea, Anca and Humbert, Nicolas and Ivanova, Anita and Mazurek, Sylwester and Novic, Marjana and Ward, Thomas R.. (2006) Artificial transfer hydrogenases based on the biotin-(strept)avidin technology : fine tuning the selectivity by saturation mutagenesis of the host protein. Journal of the American Chemical Society, 128 (25). pp. 8320-8328.

Le Trong, Isolde and Humbert, Nicolas and Ward, Thomas R. and Stenkamp, Ronald E.. (2006) Crystallographic Analysis of a Full-length Streptavidin with Its C-terminal Polypeptide Bound in the Biotin Binding Site. Journal of Molecular Biology, 356 (3). pp. 738-745.

Thomas, Christophe M. and Letondor, Christophe and Humbert, Nicolas and Ward, Thomas R.. (2005) Aqueous oxidation of alcohols catalyzed by artificial metalloenzymes based on the biotin-avidin technology. Journal of Organometallic Chemistry, 690 (20). pp. 4488-4491.

Letondor, Christophe and Humbert, Nicolas and Ward, Thomas R.. (2005) Artificial metalloenzymes based on biotin-avidin technology for the enantioselective reduction of ketones by transfer hydrogenation. Proceedings of the National Academy of Sciences, 102 (13). pp. 4683-4687.

Humbert, Nicolas and Zocchi, Andrea and Ward, Thomas R.. (2005) Electrophoretic behavior of streptavidin complexed to a biotinylated probe: A functional screening assay for biotin-binding proteins. Electrophoresis, 26 (1). pp. 47-52.

Klein, Gerard and Humbert, Nicolas and Gradinaru, Julieta and Ivanova, Anita and Gilardoni, Francois and Rusbandi, Untung E. and Ward, Thomas R.. (2005) Tailoring the Active Site of Chemzymes by Using a Chemogenetic-Optimization Procedure: Towards Substrate-Specific Artificial Hydrogenases Based on the Biotin–Avidin Technology. Angewandte Chemie International Edition, 44 (47). pp. 7764-7767.

Skander, Myriem and Humbert, Nicolas and Collot, Jerome and Gradinaru, Julieta and Klein, Gerard and Loosli, Andreas and Sauser, Jerome and Zocchi, Andrea and Gilardoni, Francois and Ward, Thomas R.. (2004) Artificial Metalloenzymes:  (Strept)avidin as Host for Enantioselective Hydrogenation by Achiral Biotinylated Rhodium−Diphosphine Complexes. Journal of the American Chemical Society, 126 (44). pp. 14411-14418.

Collot, Jerome and Humbert, Nicolas and Skander, Myriem and Klein, Gerard and Ward, Thomas R.. (2004) Artificial metalloenzymes for enantioselective catalysis : the phenomenon of protein accelerated catalysis. Journal of Organometallic Chemistry, 689 (25). pp. 4868-4871.

Collot, Jerome and Gradinaru, Julieta and Humbert, Nicolas and Skander, Myriem and Zocchi, Andrea and Ward, Thomas R.. (2003) Artificial metalloenzymes for enantioselective catalysis based on biotin-avidin. Journal of the American Chemical Society, 125 (30). pp. 9030-9031.

Zocchi, Andrea and Humbert, Nicolas and Berta, Temugin and Ward, Thomas R.. (2003) Efficient expression and mutation of avidin and streptavidin as host proteins for enantioselective catalysis. Chimia, 57 (10). pp. 589-592.

Book Section

Humbert, Nicolas and Ward, Thomas R.. (2008) Functionality screen of streptavidin mutants by non-denaturing SDS-PAGE using biotin-4-fluorescein. In: Avidin-Biotin Interactions: Methods and Applications, 418. Totowa, NJ 07512 USA, pp. 63-71.

Humbert, Nicolas and Schormann, Peter and Zocchi, Andrea and Neuhaus, Jean-Marc and Ward, Thomas R.. (2008) High-yield production and purification of recombinant T7-tag mature streptavidin in glucose-stressed E-coli. In: Avidin-Biotin Interactions: Methods and Applications, 418. Totowa, NJ, pp. 101-110.