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Real-time and automated monitoring of antischistosomal drug activity profiles for screening of compound libraries

Ravaynia, P. S. and Biendl, S. and Grassi, F. and Keiser, J. and Hierlemann, A. and Modena, M. M.. (2022) Real-time and automated monitoring of antischistosomal drug activity profiles for screening of compound libraries. iScience, 25 (4). p. 104087.

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Official URL: https://edoc.unibas.ch/90791/

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Abstract

Schistosomiasis is a neglected tropical disease that affects over 200 million people annually. As the antischistosomal drug pipeline is currently empty, repurposing of compound libraries has become a source for accelerating drug development, which demands the implementation of high-throughput and efficient screening strategies. Here, we present a parallelized impedance-based platform for continuous and automated viability evaluation of Schistosoma mansoni schistosomula in 128 microwells during 72 h to identify antischistosomal hits in vitro. By initially screening 57 repurposed compounds against larvae, five drugs are identified, which reduce parasite viability by more than 70%. The activity profiles of the selected drugs are then investigated via real-time dose-response monitoring, and four compounds reveal high potency and rapid action, which renders them suitable candidates for follow-up tests against adult parasites. The study shows that our device is a reliable tool for real-time drug screening analysis of libraries to identify new promising therapeutics against schistosomiasis.
Faculties and Departments:09 Associated Institutions > Swiss Tropical and Public Health Institute (Swiss TPH)
09 Associated Institutions > Swiss Tropical and Public Health Institute (Swiss TPH) > Department of Medical Parasitology and Infection Biology (MPI) > Helminth Drug Development (Keiser)
UniBasel Contributors:Biendl, Stefan and Keiser, Jennifer
Item Type:Article, refereed
Article Subtype:Research Article
ISSN:2589-0042 (Electronic)2589-0042 (Linking)
Note:Publication type according to Uni Basel Research Database: Journal article
Language:English
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Last Modified:27 Dec 2022 21:08
Deposited On:27 Dec 2022 21:08

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