Timinao, L. and Vinit, R. and Katusele, M. and Koleala, T. and Nate, E. and Czeher, C. and Burkot, T. R. and Schofield, L. and Felger, I. and Mueller, I. and Laman, M. and Robinson, L. J. and Karl, S.. (2021) Infectivity of symptomatic malaria patients to Anopheles farauti colony mosquitoes in Papua New Guinea. Front Cell Infect Microbiol, 11. p. 771233.
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Abstract
Plasmodium transmission from humans to mosquitoes is an understudied bottleneck in the transmission of malaria. Direct membrane feeding assays (DMFA) allow detailed malaria transmission studies from humans to mosquitoes. Especially for Plasmodium vivax, which cannot be cultured long-term under laboratory conditions, implementation of DMFAs requires proximity to P. vivax endemic areas. In this study, we investigated the infectivity of symptomatic Plasmodium infections to Anopheles farauti colony mosquitoes in Papua New Guinea (PNG). A total of 182 DMFAs were performed with venous blood collected from rapid diagnostic test (RDT) positive symptomatic malaria patients and subsequently analysed by light microscopy and quantitative real time polymerase chain reaction (qPCR). DMFAs resulted in mosquito infections in 20.9% (38/182) of cases. By light microscopy and qPCR, 10 - 11% of P. falciparum and 32 - 44% of P. vivax positive individuals infected An. farauti. Fifty-eight percent of P. vivax and 15% of P. falciparum gametocytaemic infections infected An farauti.
Faculties and Departments: | 09 Associated Institutions > Swiss Tropical and Public Health Institute (Swiss TPH) 09 Associated Institutions > Swiss Tropical and Public Health Institute (Swiss TPH) > Former Units within Swiss TPH > Molecular Diagnostics (Felger) |
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UniBasel Contributors: | Felger, Ingrid |
Item Type: | Article, refereed |
Article Subtype: | Research Article |
ISSN: | 2235-2988 (Electronic)2235-2988 (Linking) |
Note: | Publication type according to Uni Basel Research Database: Journal article |
Language: | English |
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Last Modified: | 21 Dec 2022 12:39 |
Deposited On: | 21 Dec 2022 12:39 |
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