Wang, Yu and Patil, Kiran M. and Yan, Shuanghong and Zhang, Panke and Guo, Weiming and Wang, Yuqin and Chen, Hong-Yuan and Gillingham, Dennis and Huang, Shuo. (2019) Nanopore Sequencing Accurately Identifies the Mutagenic DNA Lesion O; 6; -Carboxymethyl Guanine and Reveals Its Behavior in Replication. Angewandte Chemie International Edition, 58 (25). pp. 8432-8436.
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Official URL: https://edoc.unibas.ch/74577/
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Abstract
O; 6; -carboxymethylguanine (O; 6; -CMG) is a highly mutagenic alkylation product of DNA, triggering transition mutations relevant to gastrointestinal cancer. However, precise localization of a single O; 6; -CMG with conventional sequencing platforms is challenging. Here nanopore sequencing (NPS), which directly senses single DNA bases according to their physiochemical properties, was employed to detect O; 6; -CMG. A unique O; 6; -CMG signal was observed during NPS and a single-event call accuracy of >95 % was achieved. Moreover, O; 6; -CMG was found to be a replication obstacle for Phi29 DNA polymerase (Phi29 DNAP), suggesting this lesion could cause DNA sequencing biases in next generation sequencing (NGS) approaches.
Faculties and Departments: | 05 Faculty of Science > Departement Chemie > Chemie > Organische Chemie (Gillingham) |
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UniBasel Contributors: | Gillingham, Dennis |
Item Type: | Article, refereed |
Article Subtype: | Research Article |
Publisher: | Wiley |
ISSN: | 1433-7851 |
e-ISSN: | 1521-3773 |
Note: | Publication type according to Uni Basel Research Database: Journal article |
Language: | English |
Identification Number: |
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edoc DOI: | |
Last Modified: | 14 May 2020 09:39 |
Deposited On: | 21 Jan 2020 12:17 |
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