Deplancke, Bart and Mukhopadhyay, Arnab and Ao, Wanyuan and Elewa, Ahmed M. and Grove, Christian A. and Martinez, Natalia J. and Sequerra, Reynaldo and Doucette-Stamm, Lynn and Reece-Hoyes, John S. and Hope, Ian A. and Tissenbaum, Heidi A. and Mango, Susan E. and Walhout, Albertha J. M.. (2006) A gene-centered C. elegans protein-DNA interaction network. Cell, 125 (6). pp. 1193-1205.
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Official URL: https://edoc.unibas.ch/70584/
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Abstract
Transcription regulatory networks consist of physical and functional interactions between transcription factors (TFs) and their target genes. The systematic mapping of TF-target gene interactions has been pioneered in unicellular systems, using "TF-centered" methods (e.g., chromatin immunoprecipitation). However, metazoan systems are less amenable to such methods. Here, we used "gene-centered" high-throughput yeast one-hybrid (Y1H) assays to identify 283 interactions between 72 C. elegans digestive tract gene promoters and 117 proteins. The resulting protein-DNA interaction (PDI) network is highly connected and enriched for TFs that are expressed in the digestive tract. We provide functional annotations for approximately 10% of all worm TFs, many of which were previously uncharacterized, and find ten novel putative TFs, illustrating the power of a gene-centered approach. We provide additional in vivo evidence for multiple PDIs and illustrate how the PDI network provides insights into metazoan differential gene expression at a systems level.
Faculties and Departments: | 05 Faculty of Science > Departement Biozentrum > Growth & Development > Cell and Developmental Biology (Mango) |
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UniBasel Contributors: | Mango, Susan Elizabeth |
Item Type: | Article, refereed |
Article Subtype: | Research Article |
Publisher: | Cell Press |
ISSN: | 0092-8674 |
e-ISSN: | 1097-4172 |
Note: | Publication type according to Uni Basel Research Database: Journal article |
Identification Number: |
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Last Modified: | 13 Nov 2020 10:00 |
Deposited On: | 13 Nov 2020 10:00 |
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