Han, Zhenbo and Riefler, Gary M. and Saam, Jennifer R. and Mango, Susan E. and Schumacher, Jill M.. (2005) The C. elegans Tousled-like kinase contributes to chromosome segregation as a substrate and regulator of the Aurora B kinase. Current Biology, 15 (10). pp. 894-904.
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Official URL: https://edoc.unibas.ch/70582/
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Abstract
The Aurora kinases control multiple aspects of mitosis, among them centrosome maturation, spindle assembly, chromosome segregation, and cytokinesis. Aurora activity is regulated in part by a subset of Aurora substrates that, once phosphorylated, can enhance Aurora kinase activity. Aurora A substrate activators include TPX2 and Ajuba, whereas the only known Aurora B substrate activator is the chromosomal passenger INCENP.; We report that the C. elegans Tousled kinase TLK-1 is a second substrate activator of the Aurora B kinase AIR-2. Tousled kinase (Tlk) expression and activity have been linked to ongoing DNA replication, and Tlk can phosphorylate the chromatin assembly factor Asf. Here, we show that TLK-1 is phosphorylated by AIR-2 during prophase/prometaphase and that phosphorylation increases TLK-1 kinase activity in vitro. Phosphorylated TLK-1 increases AIR-2 kinase activity in a manner that is independent of TLK-1 kinase activity but depends on the presence of ICP-1/INCENP. In vivo, TLK-1 and AIR-2 cooperate to ensure proper mitotic chromosome segregation.; The C. elegans Tousled kinase TLK-1 is a substrate and activator of the Aurora B kinase AIR-2. These results suggest that Tousled kinases have a previously unrecognized role in mitosis and that Aurora B associates with discrete regulatory complexes that may impart distinct substrate specificities and functions to the Aurora B kinase.
Faculties and Departments: | 05 Faculty of Science > Departement Biozentrum > Growth & Development > Cell and Developmental Biology (Mango) |
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UniBasel Contributors: | Mango, Susan Elizabeth |
Item Type: | Article, refereed |
Article Subtype: | Research Article |
Publisher: | Cell Press |
ISSN: | 0960-9822 |
e-ISSN: | 1879-0445 |
Note: | Publication type according to Uni Basel Research Database: Journal article |
Related URLs: | |
Identification Number: |
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Last Modified: | 13 Nov 2020 09:56 |
Deposited On: | 13 Nov 2020 09:56 |
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