edoc

Molecular basis of mRNA localization and translation control

Jia, Min. Molecular basis of mRNA localization and translation control. 2018, Doctoral Thesis, University of Basel, Faculty of Science.

[img] PDF
Restricted to Repository staff only until 1 January 2020.

140Mb

Official URL: http://edoc.unibas.ch/diss/DissB_12982

Downloads: Statistics Overview

Abstract

A common mechanism for achieving mRNA localization is the assembly of RNA-protein complexes that are transported by molecular motors along the cytoskeleton to their cellular destination. My project is to investigate how the IMP family of RNA-binding proteins are able to assemble RNA granules and mediate transport. IMP proteins belong to a conserved protein family, which contain multiple RNA-binding domains. Using a variety of biochemical and structural techniques, I determined the molecular basis of RNA recognition for the IMP3 RRM1+RRM2 domain. While this domain has only modest sequence specificity and affinity in RNA binding, these interactions maybe important in the context of the full-length protein. Additionally, I also developed a single-molecule RNA mobility tethering assay to investigate the role of the IMP RRM domains in RNA transport. Interestingly, tethering of the IMP RRM domains to transcripts results in reduced movement of RNA dots in HeLa cells. The motor responsible for this anchoring, however, has not yet been identified.
Advisors:Gasser, Susan M. and Chao, Jeffrey and Niessing, Dierk
Faculties and Departments:09 Associated Institutions > Friedrich Miescher Institut FMI > Quantitative Biology > Nuclear organization in development and genome stability (Gasser)
Item Type:Thesis
Thesis Subtype:Doctoral Thesis
Thesis no:12982
Thesis status:Complete
Bibsysno:Link to catalogue
Number of Pages:1 Online-Ressource (154 Seiten)
Language:English
Identification Number:
Last Modified:05 Apr 2019 08:40
Deposited On:05 Apr 2019 08:16

Repository Staff Only: item control page