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Visual histological grading system for the evaluation of in vitro-generated neocartilage

Grogan, Shawn Patrick and Barbero, Andrea and Winkelmann, Verena and Rieser, Franz and Fitzsimmons, James S. and O`Driscoll, Shawn and Martin, Ivan and Mainil-Varlet, Pierre. (2006) Visual histological grading system for the evaluation of in vitro-generated neocartilage. Tissue Engineering, Vol. 12, H. 8. pp. 2141-2149.

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Official URL: http://edoc.unibas.ch/dok/A5249012

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Abstract

Here we present the development of a visual evaluation system for routine assessment of in vitro-engineered cartilaginous tissue. Neocartilage was produced by culturing human articular chondrocytes in pellet culture systems or in a scaffold-free bioreactor system. All engineered tissues were embedded in paraffin and were sectioned and stained with Safranin O-fast green. The evaluation of each sample was broken into 3 categories (uniformity and intensity of Safranin O stain, distance between cells/amount of matrix produced, and cell morphology), and each category had 4 components with a score ranging from 0 to 3. Three observers evaluated each sample, and the new system was independently tested against an objective computer-based histomorphometry system. Pellets were also assessed biochemically for glycosaminoglycan (GAG) content. Pellet histology scores correlated significantly with GAG contents and were in agreement with the computer-based histomorphometry system. This system allows a valid and rapid assessment of in vitro-generated cartilaginous tissue that has a relevant association with objective parameters indicative of cartilage quality.
Faculties and Departments:03 Faculty of Medicine > Departement Biomedizin > Department of Biomedicine, University Hospital Basel > Tissue Engineering (Martin)
UniBasel Contributors:Martin, Ivan
Item Type:Article, refereed
Article Subtype:Research Article
Bibsysno:Link to catalogue
Publisher:Mary Ann Liebert
ISSN:1076-3279
Note:Publication type according to Uni Basel Research Database: Journal article. This is a copy of an article published in the Tissue engineering © 2007 [copyright Mary Ann Liebert, Inc.]; Tissue engineering is available online at: http://online.liebertpub.com.
Language:English
Identification Number:
Last Modified:31 Dec 2015 10:44
Deposited On:22 Mar 2012 13:36

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