Lee, Ki Jeong and Perez, Mar and Pinschewer, Daniel D. and de la Torre, Juan Carlos. (2002) Identification of the lymphocytic choriomeningitis virus (LCMV) proteins required to rescue LCMV RNA analogs into LCMV-like particles. Journal of Virology, 76 (12). pp. 6393-6397.
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Abstract
We have used a reverse genetic approach to identify the viral proteins required for packaging and assembly of the prototypic arenavirus lymphocytic choriomeningitis virus (LCMV). Plasmids encoding individual LCMV proteins under the control of an RNA polymerase II promoter were cotransfected with a plasmid containing an LCMV minigenome (MG). Intracellular synthesis of the LCMV MG was driven by T7 RNA polymerase whose expression was also mediated by a Pol II promoter. The supernatant from transfected cells was passaged onto fresh cells that were subsequently infected with LCMV to provide the minimal viral trans-acting factors, NP and L, that are required for LCMV MG RNA replication and expression. Reconstitution of LCMV-specific packaging and passage was detected by expression of the chloramphenicol acetyl transferase (CAT) reporter gene present in the MG. NP and L did not direct detectable levels of MG passage. Addition of Z and GP resulted in high levels of passage of CAT activity, which could be prevented by LCMV neutralizing antibodies. Passage of LCMV MG was inhibited by omission of either GP or Z.
Faculties and Departments: | 03 Faculty of Medicine > Departement Biomedizin > Division of Medical Microbiology > Experimental Virology (Pinschewer) |
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UniBasel Contributors: | Pinschewer, Daniel |
Item Type: | Article, refereed |
Article Subtype: | Research Article |
Publisher: | American Society of Microbiology |
ISSN: | 0022-538X |
e-ISSN: | 1098-5514 |
Note: | Publication type according to Uni Basel Research Database: Journal article |
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Last Modified: | 16 Dec 2020 10:53 |
Deposited On: | 16 Dec 2020 10:53 |
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