A plasmid collection for PCR-based gene targeting in the filamentous ascomycete Ashbya gossypii

Kaufmann, A.. (2009) A plasmid collection for PCR-based gene targeting in the filamentous ascomycete Ashbya gossypii. Fungal Genetics and Biology, Vol. 46, Issue 8. pp. 595-603.

Full text not available from this repository.

Official URL: http://edoc.unibas.ch/dok/A5257582

Downloads: Statistics Overview


PCR-based gene targeting with heterologous markers is an efficient method to delete genes, generate gene fusions, and modulate gene expression. For the yeasts Saccharomyces cerevisiae and Schizosaccharomyces pombe, several plasmid collections are available covering a wide range of tags and markers. For several reasons, many of these cassettes cannot be used in the filamentous ascomycete Ashbya gossypii. This article describes the construction of 93 heterologous modules for C- and N-terminal tagging and promoter replacements in A.gossypii. The performance of 12 different fluorescent tags was evaluated by monitoring their brightness, detectability, and photostability when fused to the myosin light-chain protein Mlc2. Furthermore, the thiamine-repressible S.cerevisiae THI13 promoter was established to regulate gene expression in A.gossypii. This collection will help accelerate analysis of gene function in A.gossypii and in other ascomycetes where S.cerevisiae promoter elements are functional.
Faculties and Departments:05 Faculty of Science > Departement Biozentrum > Former Organization Units Biozentrum > Applied Microbiology (Philippsen)
UniBasel Contributors:Philippsen, Peter
Item Type:Article, refereed
Article Subtype:Research Article
Note:Publication type according to Uni Basel Research Database: Journal article
Last Modified:22 Mar 2012 14:23
Deposited On:22 Mar 2012 13:32

Repository Staff Only: item control page