Gasser, R. and Hauri, H. P. and Meyer, U. A.. (1982) The turnover of cytochrome P450b. FEBS letters, Vol. 147, H. 2. pp. 239-242.
Full text not available from this repository.
Official URL: http://edoc.unibas.ch/dok/A5257844
Downloads: Statistics Overview
Abstract
The turnover of the heme and apocytochrome moieties of phenobarbital-inducible microsomal cytochrome P450 (P450b) was investigated. Adult male Sprague-Dawley rats were treated with phenobarbital for 5 days and injected with [35S]methionine and the heme precursor delta-[3H]-aminolevulinic acid. P450b was isolated by immunoprecipitation and quantitated by rocket immunoelectrophoresis. The isotope disappearance curves revealed a mean half-life (T1/2) of 12.4 h for the heme moiety and a T1/2 of 19.1 h for the apoprotein moiety of P450b. The apparently slower catabolic rate of the apoprotein may be due to reutilization of [35S]methionine and does not exclude synchronous turnover of the two moieties. Our data are consistent with the kinetics of the drug-mediated induction of cytochrome P450b.
| Faculties and Departments: | 05 Faculty of Science > Departement Biozentrum > Former Organization Units Biozentrum > Pharmacology/Neurobiology (Hauri) |
|---|---|
| UniBasel Contributors: | Hauri, Hans-Peter |
| Item Type: | Article, refereed |
| Article Subtype: | Research Article |
| Publisher: | Elsevier Science |
| ISSN: | 0014-5793 |
| Note: | Publication type according to Uni Basel Research Database: Journal article |
| Last Modified: | 22 Mar 2012 14:23 |
| Deposited On: | 22 Mar 2012 13:32 |
Repository Staff Only: item control page