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Biotin-independent strains of Escherichia coli for enhanced streptavidin production

Jeschek, Markus and Bahls, Maximilian O. and Schneider, Veronika and Marlière, Philippe and Ward, Thomas R. and Panke, Sven. (2017) Biotin-independent strains of Escherichia coli for enhanced streptavidin production. Metabolic Engineering, 40. pp. 33-40.

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Abstract

Biotin is an archetypal vitamin used as cofactor for carboxylation reactions found in all forms of life. However, biotin biosynthesis is an elaborate multi-enzymatic process and metabolically costly. Moreover, many industrially relevant organisms are incapable of biotin synthesis resulting in the requirement to supplement defined media. Here we describe the creation of biotin-independent strains of Escherichia coli and Corynebacterium glutamicum through installation of an optimized malonyl-CoA bypass, which re-routes natural fatty acid synthesis, rendering the previously essential vitamin completely obsolete. We utilize biotin-independent E. coli for the production of the high-value protein streptavidin which was hitherto restricted because of toxic effects due to biotin depletion. The engineered strain revealed significantly improved streptavidin production resulting in the highest titers and productivities reported for this protein to date.
Faculties and Departments:05 Faculty of Science > Departement Chemie > Chemie > Bioanorganische Chemie (Ward)
UniBasel Contributors:Ward, Thomas R. R.
Item Type:Article, refereed
Article Subtype:Research Article
Publisher:Elsevier
ISSN:1096-7176
e-ISSN:1096-7184
Note:Publication type according to Uni Basel Research Database: Journal article
Language:English
Identification Number:
Last Modified:17 Aug 2018 13:01
Deposited On:22 Jan 2018 10:06

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