Eudesmanes sesquiterpenes from Verbesina lanata with inhibitory activity against grapevine downy mildew

Ramseyer, Justine and Thuerig, Barbara and De Mieri, Maria and Schärer, Hans-Jakob and Oberhänsli, Thomas and Gupta, Mahabir P. and Tamm, Lucius and Hamburger, Matthias and Potterat, Olivier. (2017) Eudesmanes sesquiterpenes from Verbesina lanata with inhibitory activity against grapevine downy mildew. Journal of Natural Products, 80 (12). pp. 3296-3304.

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Official URL: http://edoc.unibas.ch/58715/

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An in-house library of more than 3000 extracts of plant and fungal origin was screened against some major plant pathogens. As one of the hits, an ethyl acetate extract from inflorescences of Verbesina lanata showed significant inhibitory activity in vitro against grapevine downy mildew (Plasmopara viticola), with a MIC100 value of 35 μg/mL. An emulsifiable concentrate formulation with 50 mg/g of the extract was developed for in vivo evaluation. A suspension of the formulation containing 1 mg/mL of extract lowered leaf surface infection of grapevine seedling by 82% compared to the nontreated control. With the aid of HPLC-based activity profiling, the antifungal activity was correlated with a series of lipophilic compounds. Preparative isolation by a combination of chromatographic techniques afforded 16 eudesmane sesquiterpenes including eight new congeners. Nine compounds were obtained in sufficient quantities to be tested in vitro and were found to inhibit the zoospore activity of P. viticola with MIC100 values ranging from 4 to 50 μg/mL. The two major compounds, 6β-cinnamoyloxy-4β,9β,15-trihydroxyeudesmane (9) and 6β-cinnamoyloxy-1β,15-dihydroxyeudesm-4-en-3-one (13), showed MIC100 values of 5 and 31 μg/mL, respectively.
Faculties and Departments:05 Faculty of Science > Departement Pharmazeutische Wissenschaften > Pharmazie > Pharmazeutische Biologie (Hamburger)
UniBasel Contributors:Hamburger, Matthias and Ramseyer, Justine and Potterat, Olivier and de Mieri, Maria
Item Type:Article, refereed
Article Subtype:Research Article
Publisher:American Chemical Society
Note:Publication type according to Uni Basel Research Database: Journal article
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Last Modified:13 Jun 2018 09:44
Deposited On:13 Jun 2018 09:44

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