Structure and function of a novel antioxidant peptide from the skin of tropical frogs

Barbosa, Eder Alves and Oliveira, Ana and Plácido, Alexandra and Socodato, Renato and Portugal, Camila C. and Mafud, Ana Carolina and Ombredane, Alicia S. and Moreira, Daniel C. and Vale, Nuno and Bessa, Lucinda J. and Joanitti, Graziella A. and Alves, Cláudia and Gomes, Paula and Delerue-Matos, Cristina and Mascarenhas, Yvonne Primerano and Marani, Mariela M. and Relvas, João B. and Pintado, Manuela and Leite, José Roberto S. A.. (2017) Structure and function of a novel antioxidant peptide from the skin of tropical frogs. Free radical biology & medicine, 115. pp. 68-79.

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Official URL: http://edoc.unibas.ch/57498/

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The amphibian skin plays an important role protecting the organism from external harmful factors such as microorganisms or UV radiation. Based on biorational strategies, many studies have investigated the cutaneous secretion of anurans as a source of bioactive molecules. By a peptidomic approach, a novel antioxidant peptide (AOP) with in vitro free radical scavenging ability was isolated from Physalaemus nattereri. The AOP, named antioxidin-I, has a molecular weight [M+H]+ = 1543.69Da and a TWYFITPYIPDK primary amino acid sequence. The gene encoding the antioxidin-I precursor was expressed in the skin tissue of three other Tropical frog species: Phyllomedusa tarsius, P. distincta and Pithecopus rohdei. cDNA sequencing revealed highly homologous regions (signal peptide and acidic region). Mature antioxidin-I has a novel primary sequence with low similarity compared with previously described amphibian's AOPs. Antioxidin-I adopts a random structure even at high concentrations of hydrophobic solvent, it has poor antimicrobial activity and poor performance in free radical scavenging assays in vitro, with the exception of the ORAC assay. However, antioxidin-I presented a low cytotoxicity and suppressed menadione-induced redox imbalance when tested with fibroblast in culture. In addition, it had the capacity to substantially attenuate the hypoxia-induced production of reactive oxygen species when tested in hypoxia exposed living microglial cells, suggesting a potential neuroprotective role for this peptide.
Faculties and Departments:09 Associated Institutions > Swiss Tropical and Public Health Institute (Swiss TPH)
09 Associated Institutions > Swiss Tropical and Public Health Institute (Swiss TPH) > Department of Medical Parasitology and Infection Biology (MPI) > Helminth Drug Development (Keiser)
UniBasel Contributors:Mafud, Ana
Item Type:Article, refereed
Article Subtype:Research Article
Publisher:Pergamon Press
Note:Publication type according to Uni Basel Research Database: Journal article
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Last Modified:21 Dec 2017 12:41
Deposited On:21 Dec 2017 12:41

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