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Anti-melanocortin-4 receptor autoantibodies in obesity

Peter, J. C. and Bekel, A. and Lecourt, A. C. and Zipfel, G. and Eftekhari, P. and Nesslinger, M. and Breidert, M. and Muller, S. and Kessler, L. and Hofbauer, K. G.. (2009) Anti-melanocortin-4 receptor autoantibodies in obesity. Journal of clinical endocrinology and metabolism, Vol. 94, no. 3. pp. 793-800.

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Official URL: http://edoc.unibas.ch/dok/A5258605

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Abstract

Background: The melanocortin-4 receptor (MC4R) is part of an important pathway regulating energy balance. Here we report the existence of autoantibodies (autoAbs) against the MC4R in sera of obese patients. Methods: The autoAbs were detected after screening of 216 patients' sera by using direct and inhibition ELISA with an N-terminal sequence of the MC4R. Binding to the native MC4R was evaluated by flow cytometry and pharmacological effects by measuring adenylyl cyclase activity. Results: Positive results in all tests were obtained in patients with overweight or obesity (prevalence: 3.6%) but not in normal weight patients. The selective binding properties of anti-MC4R autoAbs were confirmed by surface plasmon resonance and by immunoprecipitation with the native MC4R. Finally it was demonstrated that these autoAbs increased food intake in rats after passive transfer via intracerebroventricular injection. Conclusion: These observations suggest that inhibitory anti-MC4R autoAbs might contribute to the development of obesity in a small subpopulation of patients.
Faculties and Departments:05 Faculty of Science > Departement Biozentrum > Former Organization Units Biozentrum > Pharmacology/Neurobiology (Hofbauer)
03 Faculty of Medicine > Departement Biomedizin > Former Units at DBM > Angewandte Pharmakologie (Hofbauer)
UniBasel Contributors:Hofbauer, Karl G.
Item Type:Article, refereed
Article Subtype:Research Article
Bibsysno:Link to catalogue
Publisher:Thomas
ISSN:0021-972X
Note:Publication type according to Uni Basel Research Database: Journal article
Language:English
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Last Modified:31 Dec 2015 10:43
Deposited On:22 Mar 2012 13:31

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