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Three-Dimensional Imaging of Biological Tissue by Cryo X-Ray Ptychography

Shahmoradian, S. H. and Tsai, E. H. R. and Diaz, A. and Guizar-Sicairos, M. and Raabe, J. and Spycher, L. and Britschgi, M. and Ruf, A. and Stahlberg, H. and Holler, M.. (2017) Three-Dimensional Imaging of Biological Tissue by Cryo X-Ray Ptychography. Scientific Reports, 7 (1). p. 6291.

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Official URL: http://edoc.unibas.ch/56288/

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Abstract

High-throughput three-dimensional cryogenic imaging of thick biological specimens is valuable for identifying biologically- or pathologically-relevant features of interest, especially for subsequent correlative studies. Unfortunately, high-resolution imaging techniques at cryogenic conditions often require sample reduction through sequential physical milling or sectioning for sufficient penetration to generate each image of the 3-D stack. This study represents the first demonstration of using ptychographic hard X-ray tomography at cryogenic temperatures for imaging thick biological tissue in a chemically-fixed, frozen-hydrated state without heavy metal staining and organic solvents. Applied to mammalian brain, this label-free cryogenic imaging method allows visualization of myelinated axons and sub-cellular features such as age-related pigmented cellular inclusions at a spatial resolution of ~100 nanometers and thicknesses approaching 100 microns. Because our approach does not require dehydration, staining or reduction of the sample, we introduce the possibility for subsequent analysis of the same tissue using orthogonal approaches that are expected to yield direct complementary insight to the biological features of interest.
Faculties and Departments:05 Faculty of Science > Departement Biozentrum > Structural Biology & Biophysics > Structural Biology (Stahlberg)
UniBasel Contributors:Stahlberg, Henning
Item Type:Article, refereed
Article Subtype:Research Article
Publisher:Nature Publishing Group
e-ISSN:2045-2322
Note:Publication type according to Uni Basel Research Database: Journal article
Identification Number:
Last Modified:12 Oct 2017 09:59
Deposited On:12 Oct 2017 09:59

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