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Expression, Biochemistry, and Stabilization with Camel Antibodies of Membrane Proteins: Case Study of the Mouse 5-HT3 Receptor

Hassaïne, Ghérici and Deluz, Cédric and Grasso, Luigino and Wyss, Romain and Hovius, Ruud and Stahlberg, Henning and Tomizaki, Takashi and Desmyter, Aline and Moreau, Christophe and Peclinovska, Lucie and Minniberger, Sonja and Mebarki, Lamia and Li, Xiao-Dan and Vogel, Horst and Nury, Hugues. (2017) Expression, Biochemistry, and Stabilization with Camel Antibodies of Membrane Proteins: Case Study of the Mouse 5-HT3 Receptor. Methods in Molecular Biology, 1635. pp. 139-168.

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Official URL: http://edoc.unibas.ch/56287/

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Abstract

There is growing interest in the use of mammalian protein expression systems, and in the use of antibody-derived chaperones, for structural studies. Here, we describe protocols ranging from the production of recombinant membrane proteins in stable inducible cell lines to biophysical characterization of purified membrane proteins in complex with llama antibody domains. These protocols were used to solve the structure of the mouse 5-HT3 serotonin receptor but are of broad applicability for crystallization or cryo-electron microscopy projects.
Faculties and Departments:05 Faculty of Science > Departement Biozentrum > Structural Biology & Biophysics > Structural Biology (Stahlberg)
UniBasel Contributors:Stahlberg, Henning
Item Type:Article, refereed
Article Subtype:Research Article
Publisher:Humana Press
ISSN:1064-3745
e-ISSN:1940-6029
Note:Publication type according to Uni Basel Research Database: Journal article
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Last Modified:12 Oct 2017 10:10
Deposited On:12 Oct 2017 10:10

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