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Exudate collection using wound sponges - An easy, non-invasive and reliable method to explore protease activities in ulcers

Ruf, Marie-Thérèse and Andreoli, Arianna and Vujic, Gabriel and Itin, Peter and Pluschke, Gerd and Schmid, Peter. (2017) Exudate collection using wound sponges - An easy, non-invasive and reliable method to explore protease activities in ulcers. Wound Repair and Regeneration, 25 (2). pp. 320-326.

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Official URL: http://edoc.unibas.ch/55498/

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Abstract

Proteases are important for wound healing, but in excessive amounts or left uncontrolled, they may cause healing impairment or other severe wound complications. Point-of-care testing for protease activities in wounds may be useful for monitoring the effectiveness of treatment, and for early identification of wounds that potentially fail to heal. Here we describe an easy, noninvasive method to collect wound fluid for evaluating the protease milieu of wounds. Wound fluids were collected using sterile sponges applied between wound surface and normal wound dressing. Wound fluid could be easily squeezed or centrifuged out of the sponges and was tested for gelatinase (MMP-2 and MMP-9) activities by gel zymography. In addition, we measured polymorphonuclear granulocyte elastase levels by ELISA. Both gelatinases were remarkably stable in sponge derived fluids, as no significant loss was observed even when samples were stored for 3 days at room temperature. Protease levels were highly diverse amongst patients and, in some cases, showed substantial variations in the course of the treatment. The here described wound sponge approach represents a patient-friendly and reliable method to collect wound fluid for evaluating wound healing relevant biomarkers, such as matrix metalloproteinases.
Faculties and Departments:09 Associated Institutions > Swiss Tropical and Public Health Institute (Swiss TPH)
09 Associated Institutions > Swiss Tropical and Public Health Institute (Swiss TPH) > Department of Medical Parasitology and Infection Biology > Molecular Immunology (Pluschke)
UniBasel Contributors:Ruf, Marie-Thérèse and Itin, Peter and Pluschke, Gerd
Item Type:Article, refereed
Article Subtype:Research Article
Publisher:Wiley
ISSN:1067-1927
e-ISSN:1524-475X
Note:Publication type according to Uni Basel Research Database: Journal article
Identification Number:
Last Modified:16 Oct 2017 10:13
Deposited On:16 Oct 2017 10:13

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