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Identification of microRNAs of the herpesvirus family

Pfeffer, S. and Sewer, A. and Lagos-Quintana, M. and Sheridan, R. and Sander, C. and Grasser, F. A. and van Dyk, L. F. and Ho, C. K. and Shuman, S. and Chien, M. and Russo, J. J. and Ju, J. and Randall, G. and Lindenbach, B. D. and Rice, C. M. and Simon, V. and Ho, D. D. and Zavolan, M. and Tuschl, T.. (2005) Identification of microRNAs of the herpesvirus family. Nature Methods, Vol. 2, no. 4. pp. 269-276.

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Official URL: http://edoc.unibas.ch/dok/A5259669

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Abstract

Epstein-Barr virus (EBV or HHV4), a member of the human herpesvirus (HHV) family, has recently been shown to encode microRNAs (miRNAs). In contrast to most eukaryotic miRNAs, these viral miRNAs do not have close homologs in other viral genomes or in the genome of the human host. To identify other miRNA genes in pathogenic viruses, we combined a new miRNA gene prediction method with small-RNA cloning from several virus-infected cell types. We cloned ten miRNAs in the Kaposi sarcoma-associated virus (KSHV or HHV8), nine miRNAs in the mouse gammaherpesvirus 68 (MHV68) and nine miRNAs in the human cytomegalovirus (HCMV or HHV5). These miRNA genes are expressed individually or in clusters from either polymerase (pol) II or pol III promoters, and share no substantial sequence homology with one another or with the known human miRNAs. Generally, we predicted miRNAs in several large DNA viruses, and we could neither predict nor experimentally identify miRNAs in the genomes of small RNA viruses or retroviruses.
Faculties and Departments:05 Faculty of Science > Departement Biozentrum > Computational & Systems Biology > Bioinformatics (Zavolan)
UniBasel Contributors:Zavolan, Mihaela
Item Type:Article, refereed
Article Subtype:Research Article
Bibsysno:Link to catalogue
Publisher:Nature Publishing Group
ISSN:1548-7091
Note:Publication type according to Uni Basel Research Database: Journal article
Last Modified:22 Mar 2012 14:22
Deposited On:22 Mar 2012 13:30

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