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miRNA in situ hybridization in formaldehyde and EDC-fixed tissues

Pena, J. T. G. and Sohn-Lee, C. and Rouhanifard, S. H. and Ludwig, J. and Hafner, M. and Mihailovic, A. and Lim, C. and Holoch, D. and Berninger, P. and Zavolan, M. and Tuschl, T.. (2009) miRNA in situ hybridization in formaldehyde and EDC-fixed tissues. Nature Methods, Vol. 6, no. 2. pp. 139-141.

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Official URL: http://edoc.unibas.ch/dok/A5259642

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Abstract

MicroRNAs are small regulatory RNAs with many biological functions and disease associations. We showed that in situ hybridization (ISH) using conventional formaldehyde fixation results in substantial microRNA loss from mouse tissue sections, which can be prevented by fixation with 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide that irreversibly immobilizes the microRNA at its 5' phosphate. We determined optimal hybridization parameters for 130 locked nucleic acid probes by recording nucleic acid melting temperature during ISH.
Faculties and Departments:05 Faculty of Science > Departement Biozentrum > Computational & Systems Biology > Bioinformatics (Zavolan)
UniBasel Contributors:Zavolan, Mihaela
Item Type:Article, refereed
Article Subtype:Research Article
Bibsysno:Link to catalogue
ISSN:1548-7091
Note:Publication type according to Uni Basel Research Database: Journal article
Last Modified:22 Mar 2012 14:22
Deposited On:22 Mar 2012 13:30

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