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Lectin-histochemistry of rainbow trout (Oncorhynchus mykiss) gill and skin

Burkhardt-Holm, Patricia. (1997) Lectin-histochemistry of rainbow trout (Oncorhynchus mykiss) gill and skin. Histochemical Journal, 29 (11-12). pp. 893-899.

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Official URL: http://edoc.unibas.ch/52990/

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Abstract

In order to characterize the glycoconjugate residues in skin and gills of the adult rainbow trout, the binding pattern of five biotinylated lectins with different carbohydrate specificities was examined. In the skin, mucous cells revealed binding sites for PNA and SBA; filament-containing cells were additionally labelled with Con A. However, the basal cell layer showed no reaction. In the gill, subpopulations of mucous cells reacted with Con A, PNA, SBA and UEA-I. This broader spectrum of glycoconjugates in gill mucous cells compared with the epidermal mucous cells could point to the additional function of gill mucus in ion and osmoregulation. Lectin binding sites were less common in the respiratory epithelial cells of the secondary lamellae than in those of the primary lamellae. Chloride cells revealed mannose, galactose and fucose residues. Immature chloride cells, as indicated by a comparison with Na+/K+ ATPase immunolabelling, reacted with Con A; subpopulations of them reacted with PNA, SBA and UEA-I. The results form the basis for further investigations in which these cell populations can be analysed under different environmental conditions
Faculties and Departments:05 Faculty of Science > Departement Umweltwissenschaften > Mensch Gesellschaft Umwelt MGU > Aquatische ├ľkologie (Holm)
UniBasel Contributors:Holm, Patricia
Item Type:Article, refereed
Article Subtype:Research Article
Publisher:Springer
ISSN:0018-2214
e-ISSN:1573-6865
Note:Publication type according to Uni Basel Research Database: Journal article
Identification Number:
Last Modified:22 Nov 2017 09:51
Deposited On:22 Nov 2017 09:51

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