Optimization of DNA Hybridization Efficiency by pH-Driven Nanomechanical Bending

Zhang, Jiayun and Lang, Hans Peter and Yoshikawa, Genki and Gerber, Christoph. (2012) Optimization of DNA Hybridization Efficiency by pH-Driven Nanomechanical Bending. Langmuir, 28 (15). pp. 6494-6501.

Full text not available from this repository.

Official URL: http://edoc.unibas.ch/52143/

Downloads: Statistics Overview


The accessibility and binding affinity of DNA are two key parameters affecting the hybridization efficiency in surface-based biosensor technologies. Better accessibility will result in a higher hybridization efficiency. Often, mixed ssDNA and mercaptohexanol monolayers are used to increase the hybridization efficiency and accessibility of surface-bound oligonucleotides to complementary target DNA. Here, no mercaptohexanol monolayer was used. We demonstrate by differential microcantilever deflection measurements at different pH that the hybridization efficiency peaks between pH 7.5 and 8.5. At low pH 4.5, hydration and electrostatic forces led to tensile surface stress, implying the reduced accessibility of the bound ssDNA probe for hybridization. In contrast, at high pH 8.5, the steric interaction between neighboring ssDNA strands was decreased by higher electrostatic repulsive forces, bending the microcantilever away from gold surface to provide more space for the target DNA. Cantilever deflection scales with pH-dependent surface hybridization efficiency because of high target DNA accessibility. Hence, by changing the pH, the hybridization efficiency is adjusted.
Faculties and Departments:05 Faculty of Science > Departement Physik > Physik > Nanomechanik (Meyer)
UniBasel Contributors:Lang, Hans Peter
Item Type:Article, refereed
Article Subtype:Research Article
Publisher:American Chemical Society
Note:Publication type according to Uni Basel Research Database: Journal article
Identification Number:
Last Modified:02 Mar 2017 07:52
Deposited On:01 Mar 2017 12:52

Repository Staff Only: item control page