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Two-dimensional crystallization of the mouse serotonin 5-HT3A receptor

Rheinberger, Jan and Hassaine, Ghérici and Chami, Mohamed and Stahlberg, Henning and Vogel, Horst and Li, Xiaodan. (2017) Two-dimensional crystallization of the mouse serotonin 5-HT3A receptor. Micron, 92. pp. 19-24.

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Official URL: http://edoc.unibas.ch/51769/

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Abstract

The mouse serotonin 5-HT3A receptor is a homo-pentameric ligand-gated ion channel (pLGIC) mediating fast excitatory neurotransmission in the central nervous system. The molecular mechanism of ion permeation of 5-HT3A receptors triggered by the neurotransmitter serotonin is not yet fully understood. The recent X-ray structure of the mouse serotonin 5-HT3A receptor in complex with a stabilizing nanobody revealed for the first time the entire structure of a mammalian pLGIC in detergent. Structural information of the receptor in a lipid bilayer however is still limited primarily due to the lack of 2D crystals of the receptor in a lipid bilayer. Here we present our results on the formation and improvement of diffracting 2D crystals of the mouse 5-HT3A by limited proteolysis and addition of conformational nanobodies.
Faculties and Departments:05 Faculty of Science > Departement Biozentrum > Structural Biology & Biophysics > Structural Biology (Stahlberg)
UniBasel Contributors:Stahlberg, Henning
Item Type:Article, refereed
Article Subtype:Research Article
Publisher:Elsevier
ISSN:0968-4328
e-ISSN:1878-4291
Note:Publication type according to Uni Basel Research Database: Journal article
Identification Number:
Last Modified:12 Oct 2017 08:50
Deposited On:11 Oct 2017 10:01

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