Ketogenesis in the living rat followed by 13C-NMR spectroscopy : Infusion of [1,3-13C]octanoate

Pahl-Wostl, C. and Seelig, J.. (1987) Ketogenesis in the living rat followed by 13C-NMR spectroscopy : Infusion of [1,3-13C]octanoate. Biological Chemistry Hoppe-Seyler, 368 (3). pp. 205-214.

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Official URL: http://edoc.unibas.ch/dok/A5257497

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13C-NMR spectroscopy was used as a noninvasive approach to study the metabolism of [1,3-13C]octanoate in rat liver. Using a properly adjusted surface coil a liver selection of better than 90% was achieved in the intact animal without abdominal surgery. After infusion of [1,3-13C]octanoate via the jugular vein different patterns of metabolites were observed depending on the physiological state of the rat. In the fasted animal, the major metabolites were those of the Krebs cycle while in the diabetic animal ketogenic end products were predominant. As a fatty acid of medium chain length octanoate is imported into the inner mitochondrial space without control by the carnitine acyl transferase system. Hence, the metabolic differences observed between diabetic and fasted rats result from an intramitochondrial control mechanism. The in vivo 13C-NMR results therefore support previous biochemical in vitro studies which concluded that a major control of ketone body production occurs in the inner mitochondrial space, presumably via the redox potential of the liver. As an unexpected result, 13C-NMR provides evidence for the transitory esterification of the infused 13C-labeled octanoic acid. The corresponding 13C-NMR chemical shifts are typical for glycerides.
Faculties and Departments:05 Faculty of Science > Departement Biozentrum > Former Organization Units Biozentrum > Biophysical Chemistry (Seelig J)
UniBasel Contributors:Seelig, Joachim
Item Type:Article, refereed
Article Subtype:Research Article
Publisher:de Gruyter
Note:Publication type according to Uni Basel Research Database: Journal article
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Last Modified:14 Nov 2017 11:12
Deposited On:22 Mar 2012 13:29

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