Observation of through-hydrogen-bond (2h)J(HC') in a perdeuterated protein

Cordier, F. and Rogowski, M. and Grzesiek, S. and Bax, A.. (1999) Observation of through-hydrogen-bond (2h)J(HC') in a perdeuterated protein. Journal of magnetic resonance, Vol. 140, H. 2. pp. 510-512.

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Official URL: http://edoc.unibas.ch/dok/A5258811

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It is demonstrated that J connectivity between amide protons and hydrogen-bond-accepting carbonyl carbons can be observed in perdeuterated human ubiquitin. A selective pulse scheme is used to detect these small (2h)J(HC') interactions in the presence of the much larger through-covalent-bond (2)J(HC') and (3)J(HC') couplings. The ratio of the observed through-H-bond correlation intensity and the (2)J(HC') connectivity observed in a reference spectrum indicates (2h)J(HC') values of ca. 0.4-0.6 Hz, which are only slightly smaller than the corresponding (3h)J(NC') values. However, for technical reasons, (2h)J(HC') couplings are more difficult to measure than (3h)J(NC'). Copyright 1999 Academic Press.
Faculties and Departments:05 Faculty of Science > Departement Biozentrum > Structural Biology & Biophysics > Structural Biology (Grzesiek)
UniBasel Contributors:Grzesiek, Stephan
Item Type:Article, refereed
Article Subtype:Research Article
Publisher:Academic Press
Note:Publication type according to Uni Basel Research Database: Journal article
Last Modified:22 Mar 2012 14:22
Deposited On:22 Mar 2012 13:29

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