A genetically encoded calcium indicator for chronic in vivo two-photon imaging

Mank, Marco and Santos, Alexandre Ferrão and Direnberger, Stephan and Mrsic-Flogel, Thomas D. and Hofer, Sonja B. and Stein, Valentin and Hendel, Thomas and Reiff, Dierk F. and Levelt, Christiaan and Borst, Alexander and Bonhoeffer, Tobias and Hübener, Mark and Griesbeck, Oliver. (2008) A genetically encoded calcium indicator for chronic in vivo two-photon imaging. Nature Methods, 5 (9). pp. 805-811.

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Official URL: http://edoc.unibas.ch/49618/

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Neurons in the nervous system can change their functional properties over time. At present, there are no techniques that allow reliable monitoring of changes within identified neurons over repeated experimental sessions. We increased the signal strength of troponin C-based calcium biosensors in the low-calcium regime by mutagenesis and domain rearrangement within the troponin C calcium binding moiety to generate the indicator TN-XXL. Using in vivo two-photon ratiometric imaging, we show that TN-XXL exhibits enhanced fluorescence changes in neurons of flies and mice. TN-XXL could be used to obtain tuning curves of orientation-selective neurons in mouse visual cortex measured repeatedly over days and weeks. Thus, the genetically encoded calcium indicator TN-XXL allows repeated imaging of response properties from individual, identified neurons in vivo, which will be crucial for gaining new insights into cellular mechanisms of plasticity, regeneration and disease.
Faculties and Departments:05 Faculty of Science > Departement Biozentrum > Former Organization Units Biozentrum > Neuronal circuits and brain function (Hofer)
UniBasel Contributors:Hofer, Sonja and Mrsic-Flogel, Thomas
Item Type:Article, refereed
Article Subtype:Research Article
Publisher:Nature Publishing Group
Note:Publication type according to Uni Basel Research Database: Journal article
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Last Modified:28 Nov 2017 11:10
Deposited On:28 Nov 2017 11:10

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