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Evolution of DNA compaction in microchannels

Dootz, Rolf and Otten, Alexander and Köster, Sarah and Struth, Bernd and Pfohl, Thomas. (2006) Evolution of DNA compaction in microchannels. Journal of physics. Condensed matter, 18. pp. 639-652.

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Official URL: http://edoc.unibas.ch/46503/

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Abstract

Combining microfluidics with x-ray microdiffraction and Raman microscopy, the dynamic behaviour of soft matter, with specific consideration of the molecular structure, can be investigated. Microfluidic systems enable a reduction of sample volume and shorter reaction times. By performing experiments under continuous microflow, material damage is avoided and the influence of external stress on biomacromolecules can be analysed. The generated elongated flow induces alignment of the investigated materials, allowing for an improved structural characterization. Here, the dynamics of the compaction of DNA by polypropyleneimine dotriacontaamine dendrimers, generation 4 is studied. As a consequence of the laminar flow inside the microchannels, highly defined, diffusion-controlled compaction of the DNA occurs enabling the study of different states of the reaction during one measurement by varying the observation position in the channels. The evolution of a columnar mesophase with an in-plane square symmetry is monitored by x-ray microdiffraction and the molecular interaction between the two reactants is traced using Raman microscopy, leading to a more profound comprehension of the condensation reaction. The experimental results are in accordance with finite element method simulations of the flow and diffusion profiles in the elongated flow device.
Faculties and Departments:05 Faculty of Science > Departement Chemie > Chemie > Biophysikalische Chemie (Pfohl)
UniBasel Contributors:Pfohl, Thomas
Item Type:Article, refereed
Article Subtype:Research Article
Publisher:Institute of Physics Publishing
ISSN:0953-8984
Note:Publication type according to Uni Basel Research Database: Journal article
Identification Number:
Last Modified:19 Dec 2016 14:34
Deposited On:19 Dec 2016 14:34

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