Quantitative interaction proteomics using mass spectrometry

Wepf, Alexander and Glatter, Timo and Schmidt, Alexander and Aebersold, Ruedi and Gstaiger, Matthias . (2009) Quantitative interaction proteomics using mass spectrometry. Nature Methods, 6 (3). pp. 203-205.

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Official URL: http://edoc.unibas.ch/45924/

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We present a mass spectrometry-based strategy for the absolute quantification of protein complex components isolated through affinity purification. We quantified bait proteins via isotope-labeled reference peptides corresponding to an affinity tag sequence and prey proteins by label-free correlational quantification using the precursor ion signal intensities of proteotypic peptides generated in reciprocal purifications. We used this method to quantitatively analyze interaction stoichiometries in the human protein phosphatase 2A network.
Faculties and Departments:05 Faculty of Science > Departement Biozentrum > Services Biozentrum > Proteomics (Schmidt)
UniBasel Contributors:Schmidt, Alexander
Item Type:Article, refereed
Article Subtype:Research Article
Publisher:Nature Publishing Group
Note:Publication type according to Uni Basel Research Database: Journal article
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Last Modified:29 Nov 2017 08:55
Deposited On:29 Nov 2017 08:55

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