# The standard protein mix database: a diverse data set to assist in the production of improved Peptide and protein identification software tools

Klimek, John and Eddes, James S. and Hohmann, Laura and Jackson, Jennifer and Peterson, Amelia and Letarte, Simon and Gafken, Philip R. and Katz, Jonathan E. and Mallick, Parag and Lee, Hookeun and Schmidt, Alexander and Ossola, Reto and Eng, Jimmy K. and Aebersold, Ruedi and Martin, Daniel B. . (2008) The standard protein mix database: a diverse data set to assist in the production of improved Peptide and protein identification software tools. Journal of Proteome Research, 7 (1). pp. 96-103.

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Official URL: http://edoc.unibas.ch/45910/

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## Abstract

Tandem mass spectrometry (MS/MS) is frequently used in the identification of peptides and proteins. Typical proteomic experiments rely on algorithms such as SEQUEST and MASCOT to compare thousands of tandem mass spectra against the theoretical fragment ion spectra of peptides in a database. The probabilities that these spectrum-to-sequence assignments are correct can be determined by statistical software such as PeptideProphet or through estimations based on reverse or decoy databases. However, many of the software applications that assign probabilities for MS/MS spectra to sequence matches were developed using training data sets from 3D ion-trap mass spectrometers. Given the variety of types of mass spectrometers that have become commercially available over the last 5 years, we sought to generate a data set of reference data covering multiple instrumentation platforms to facilitate both the refinement of existing computational approaches and the development of novel software tools. We analyzed the proteolytic peptides in a mixture of tryptic digests of 18 proteins, named the ISB standard protein mix, using 8 different mass spectrometers. These include linear and 3D ion traps, two quadrupole time-of-flight platforms (qq-TOF), and two MALDI-TOF-TOF platforms. The resulting data set, which has been named the Standard Protein Mix Database, consists of over 1.1 million spectra in 150+ replicate runs on the mass spectrometers. The data were inspected for quality of separation and searched using SEQUEST. All data, including the native raw instrument and mzXML formats and the PeptideProphet validated peptide assignments, are available at http://regis-web.systemsbiology.net/PublicDatasets/.
Faculties and Departments: 05 Faculty of Science > Departement Biozentrum > Services Biozentrum > Proteomics (Schmidt) Schmidt, Alexander Article, refereed Research Article American Chemical Society 1535-3893 1535-3907 Publication type according to Uni Basel Research Database: Journal article doi: 10.1021/pr070244j 28 Nov 2017 11:19 28 Nov 2017 11:19

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